Recent appearances: “Black Widow,” “Hawkeye”

Just like her adopted sister Natasha Romanoff/Black Widow (Scarlett Johansson), Yelena is a skilled assassin who survived her childhood training in Russia’s brutal Red Room. In “Hawkeye,” she was hired by Valentina to kill Clint Barton (Jeremy Renner) for murdering Natasha, but she lets him go when she learns that Natasha sacrificed herself to save the universe in “Endgame.” Since then, she’s been grieving the death of her sister and estranged from their adopted father Alexei, aka the Red Guardian.

Recent appearances: “Captain America: Brave New World,” “The Falcon and the Winter Soldier,” “Avengers: Endgame,” “Avengers: Infinity War”

Bucky is the one member of the Thunderbolts who’s been in the MCU the longest — dating all the way back to the first “Captain America” movie in 2011. On “The Falcon and the Winter Soldier,” he finally moved on from his past as a brainwashed assassin for Hydra. In his most recent appearance in “Captain America: Brave New World,” it was revealed that he’s an incoming U.S. Congressman. Even though Bucky has gotten into politics, it seems like he hasn’t fully left his superhero life behind.

Recent appearances: “Black Panther: Wakanda Forever,” “Black Widow,” “The Falcon and the Winter Soldier”

Contessa Valentina Allegra de Fontaine is the new director of the CIA and handler for the Thunderbolts. She recruited John Walker and made him the U.S. Agent after he brutally killed a civilian in “The Falcon and the Winter Soldier”; she sent Yelena to kill Clint Barton in “Hawkeye”; and she bought Avengers Tower to turn it into her base of operations. She’s a shadowy, Nick Fury-esque character whose true motivations are unclear. She was also revealed to be the ex-wife of CIA agent Everett K. Ross (Martin Freeman) in “Black Panther: Wakanda Forever.”

Recent appearances: “Black Widow,” “What If…?”

Alexei Shostakov is the Red Guardian, the Soviet Union’s super soldier equivalent to Captain America and Yelena’s off-kilter, but loving, father figure. He claims to have fought Cap in the ’80s; since then Alexei has gotten a little out of shape, but still fights by Yelena’s side.

Recent appearances: “The Falcon and the Winter Soldier”

John Walker was introduced as a government-appointed Captain America in “The Falcon and the Winter Soldier” and given Super Soldier serum, but his reputation was tarnished after he publicly killed a civilian. After that, he created his own Cap shield and was recruited by Valentina to run covert missions as her U.S. Agent.

Recent appearances: “Ant-Man the Wasp”

Ava Starr is the spy Ghost, who can turn her body invisible and intangible for short periods of time and pass through any surface. She was first introduced in “Ant-Man and the Wasp” as a rogue assassin working for the corrupted S.H.I.E.L.D. and searching for a way to control her erratic powers. With her abilities stabilized, Ava now works for Valentina.

Recent appearances: None

In the comics, Robert “Bob” Reynolds is the Superman-esque Sentry, who has the power of flight, super strength and invulnerability. He’s also the vessel for the Void, a dark entity within Bob that feeds off negativity.

Recent appearances: “Black Widow”

Antonia Dreykov is another graduate of the Red Room, the Soviet female assassin initiative run by her father (Ray Winstone). As the masked Taskmaster, she went head to head with Yelena and Natasha in “Black Widow.” She can perfectly imitate someone’s fighting style just by watching them, so she’s mastered Cap’s shield, Hawkeye’s bow and more.

Recent appearances: None

Mel is Valentina’s assistant, helping her run the Thunderbolts and always ready with a fresh cup of coffee.